Nature Environment and Pollution Technology

Mehrdad Modaresi ¹, Masumeh Iranpour ²

Affiliations
1 Department of Animal Science, Khorasgan (Isfahan) Branch, Islamic Azad University, Isfahan, IR
2 Payame Noor University-Isfahan Center, Isfahan, IR

Abstract

Fragrant pennyroyal (Mentha pulegium) from Lamiaceae family has many medicinal properties like haematopoietic enhancement. The effect of hydro alcoholic extract of pennyroyal on blood parameters of mice was studied on little laboratory mice from Balb/C race. Fifty mice were divided in five groups: control group, Placebo (0.6 cc of normal saline), and three extract treatment groups (50, 100, and 200 mg/kg/ 2days). Peritoneum injection was used for enforcing treatments. Blood samples were taken from mice hearts. Data were analysed using SPSS program at 5% probability level. Results showed that total number of white blood cells was increased significantly in third treatment group (200 mg/kg). Red blood cells also increased significantly in this group. Haemoglobin was increased in all treatment groups but haematocrit and blood indices (MCHC, MCH, MCV) did not show any significant effect. According to the results, pennyroyal extract can strengthen immune system in 200 mg/kg dose via increase in number of white blood cells and can affect haematopoietic via increase in number of red cells.

Keywords

Mentha pulegium, Plant Extract, Blood Parameters, Little Laboratory Mice.

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Mehrdad Modaresi ¹, Delaram Dadkhah ²

Affiliations
1 Department of Agriculture, Khorasgan Branch, Islamic Azad University, Isfahan, IR
2 Payame Noor University, Isfahan Center, Isfahan, IR

Abstract

Yarrow (Achillea millefolium) means thousands leaves plant and belongs to Asteraceae family. Yarrow is a perennial plant which grows in mountainous regions of Europe, North Africa and East Asia (including North Iran and Alborz heights). This plant has many health benefits like treating problems of digestive system, respiratory system, cardiovascular system (stimulating blood circulation and lowering blood pressure), and genital-urinary system. In this study, the effect of Yarrow extract was evaluated on immunity parameters of blood serum in little female laboratory rats (Balb/C race). At first, samples were divided randomly in five groups: three treatment groups, one control group and one placebo group. Each group had eight members and all groups were kept in similar conditions. Hydroalcoholic extract was prepared in three doses including 50,100 and 200 mg/kg and was injected in peritoneum every two days for twenty days. Normal saline was injected to placebo group. All measurements were done using protein electrophoresis method. Measured parameters were albumin amount, α-1 and α-2 globulin, β globulin, gamma globulin, albumin globulin ratio, and total protein. Results showed that the amount of albumin, β globulin and total protein were increased in all three doses. α-2 were decreased in all treatments and α-1 were increased in all three doses but this increase was significant for doses 100 and 200 mg. Albumin globulin ratio was increased also in all treatments but it was significant only for 50 mg. Gamma globulin amount was increased in 50 and 100 mg but decreased a little in 200 mg. Results of this study showed that Yarrow extract caused significant changes in blood immune parameters and can affect immune system of body even in absence of antigenic factors.

Keywords

Achillea millefolium, Hydroalcoholic Extract, Immune Factors, Blood Proteins of Mice.

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Mehrdad Modaresi ¹

Affiliations
1 Department of Physiology, Islamic Azad University, Khorasgan Branch, Esfahan, IR

Abstract

Soybean is a member of Fabaceae and a species of legume native to east Asia. It is an annual plant that has been used in China for 5000 years as a food and a component of drugs. Soy contains significant amount of all the essential amino acids for humans and so is a good source of protein. Soy has an important role in improvement and treatment of some cancers such as colon, prostate and breast. A total of male mice with 30-35 g weight were bought from Razi Institute in Iran. At first the samples were kept under adaptation condition for two weeks and then randomly grouped into 4 experimental sections as: 1-Control group were feed with soy-free basic diet. 2-Nutrition containing 20% soy diet. 3-Nutrition containing 30% soy diet. 4-Nutrition containing 50% soy diet. At the end of 8 weeks of treatment blood was collected and serum stored for hormonal analysis. Results were computed with SPSS software and compared with control group. In 20% group the level of testosterone has meaningful decrease in comparison with control group, but in 50% group the level of testosterone has meaningful increase. Levels of LH in 30% and 50% groups have a meaningful increase but no significant difference was observed in FSH and weight of testicles. The number of sperms in all of the treatments have a meaningful decrease. The result of this research indicated that the 20, 30 and 50 percent soy diet had negative effect on male reproductive system in mice.

Keywords

Soybean, Phytoestrogen, Reproductive Physiology, Male Adult Mice.

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